Radioimmunoassay for Serum Thyroxine by the Antibody-Coated Test Tube Method (SPAC-T4 Kit)
نویسندگان
چکیده
منابع مشابه
Assessment of a method for measuring serum thyroxine by radioimmunoassay, with use of polyethylene glycol precipitation.
We assessed the efficacy of a new thyroxine radioimmunoassay kit (Abott) in which polyethylene glycol is used to separate bound from free hormone. Mean serum thyroxine was 88 +/- 15 (+/-SD) microgram/liter for 96 normal persons. Results for hypothyroid and hyperthyroid persons were clearly separated from those for normal individuals. Women taking oral contraceptive preparations showed variable ...
متن کاملRadioimmunoassay of thyroxine in unextracted serum, by a single-antibody technique.
A radioimmunoassay procedure for thyroxine (14) is described, for which only 25 il of serum is required. The antibody, commercially available, is produced in rabbits in response to injections of a T4-albumin conjugate. Native T4-binding proteins are denatured by heating 25 .tl of serum in 0.2 ml of a glutamate buffer in a boiling water bath. After a 1-h incubation of heat-treated serum with l12...
متن کاملSensitive radioimmunoassay of total thyroxine (T4) in horses using a simple extraction method
Most thyroid hormone determinations in animals are based on immunoassays adapted from those used to test human samples, which may not reflect the actual values of thyroid hormone in horses because of the presence of binding proteins. The aims of the present study were i) to establish a novel radioimmunoassay (RIA) using a more simple and convenient method to separate binding proteins for the me...
متن کاملDetermination of free thyroxine in serum by radioimmunoassay.
We describe a sensitive and specific radioimmunoassay for free thyroxine, directly determined in the diffusate obtained from equilibrium dialysis of serum. The intra- and interassay precisions were 7.5 +/- 0.27 and 9.1 +/- 0.58 ng/liter (mean +/- SD), respectively. The free thyroxine concentrations of normal persons and patients with thyroid disease were as follows: normal, 7.6 +/- 1.7; euthyro...
متن کاملFREE THYROXINE (fT4) ENZYME IMMUNOASSAY TEST KIT
Thyroxine, the principal thyroid hormone, circulates in blood almost completely bound to carrier proteins. The main carrier is thyroxine-binding globulin (TBG). However, only the free (unbound) portion of thyroxine is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function a...
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ژورنال
عنوان ژورنال: RADIOISOTOPES
سال: 1980
ISSN: 1884-4111,0033-8303
DOI: 10.3769/radioisotopes.29.39